Home » Uroblog » Creation of kidney organoids from human iPS cells : @Nature via @MarcGozlan

Creation of kidney organoids from human iPS cells : @Nature via @MarcGozlan

Framed Kidney Anatomy selected by Hupertan

Anatomically Correct Framed Kidney Wall Hanging par HeyPaulStudios

Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis : Nature

Les reins sont des organes d’une complexité inouïe. Leur principale fonction de nettoyer le sang des « déchets » du métabolisme est assurée par les unités de filtration appelé néphrons. Pouvoir « fabrique » des terrains organiques dans leur complexité à partir des cellules souches peut les potins te c’est une avancée fantastique dont la recherche fondamentale nécrologique. Une équipe de chercheurs australiens a publié un excellent article dans la fameuse avait vu nature qui ouvre la voie des savons ces majeures sur en médecine.

Abstract

The human kidney contains up to 2 million epithelial nephrons responsible for blood filtration. Regenerating the kidney requires the induction of the more than 20 distinct cell types required for excretion and the regulation of pH, and electrolyte and fluid balance. We have previously described the simultaneous induction of progenitors for both collecting duct and nephrons via the directed differentiation of human pluripotent stem cells. Paradoxically, although both are of intermediate mesoderm in origin, collecting duct and nephrons have distinct temporospatial origins. Here we identify the developmental mechanism regulating the preferential induction of collecting duct versus kidney mesenchyme progenitors. Using this knowledge, we have generated kidney organoids that contain nephrons associated with a collecting duct network surrounded by renal interstitium and endothelial cells. Within these organoids, individual nephrons segment into distal and proximal tubules, early loops of Henle, and glomeruli containing podocytes elaborating foot processes and undergoing vascularization. When transcription profiles of kidney organoids were compared to human fetal tissues, they showed highest congruence with first trimester human kidney. Furthermore, the proximal tubules endocytose dextran and differentially apoptose in response to cisplatin, a nephrotoxicant. Such kidney organoids represent powerful models of the human organ for future applications, including nephrotoxicity screening, disease modelling and as a source of cells for therapy.

Source : Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis : Nature : Nature Publishing Group

Thanx to  3 hil y a 3 heures for the tweet Creation of primitive kidneys (nephrons with collected duct network) from human iPS

 


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